Automated filter paper assay determination cellulase activity

Automated filter paper assay determination cellulase activity

assays and sample values are corrected for any blank values. 2. Scope. This procedure is only appropriate for the determination of FPU activity in a cellulase preparation as defined by the IUPAC procedure as outlined above. 3. References. Ghose, T.K. "Measurement of Cellulase Activities." Pure & Appl. Chem. Apr 17,  · Automated filter paper assay for determination of cellulase activity Automated filter paper assay for determination of cellulase activity Decker, Stephen; Adney, William; Jennings, Edward; Vinzant, Todd; Himmel, Michael Recent developments in molecular breeding and directed evolution have promised great Decker et al. proposed an attractive method for automated filter paper activity/assay determination, but the results obtained by these authors were not similar to those obtained with the standard method proposed by Mandels et al.. The standard method, performed manually, resulted in a FPA of FPU/mL in the commercial cellulase preparation

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Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page. Applied Biochemistry and Biotechnology01 JanDOI: Read article at publisher's site DOI : Himmel MEAbbas CABaker JOBayer EABomble YJBrunecky RChen XFelby CJeoh T automated filter paper assay determination cellulase activity, Kumar RMcCleary BVPletschke BITucker MPWyman CEDecker SR.

Biotechnol Biofuels, 28 Nov Cited by: 2 articles PMID: PMCID: PMC Anders NSchelden MRoth SSpiess AC. Anal Bioanal Chem2002 Jun Cited by: 0 articles PMID: Mühlmann MKunze MRibeiro JGeinitz BLehmann CSchwaneberg UCommandeur UBüchs J. J Biol Eng, 07 Jan Cited by: 12 articles PMID: PMCID: PMC Sharma ATewari RRana SSSoni RSoni SK, automated filter paper assay determination cellulase activity.

Appl Biochem Biotechnol811 Apr Cited by: 25 articles PMID: Deng KTakasuka TEBianchetti CMBergeman LFautomated filter paper assay determination cellulase activity, Adams PDNorthen TRFox BG. Front Bioeng Biotechnol, automated filter paper assay determination cellulase activity Oct Cited by: 4 articles PMID: PMCID: PMC To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Luciano Silveira MHRau MPinto da Silva Bon EAndreaus J. Enzyme Microb Technol51 531 Jul Cited by: 3 articles PMID: automated filter paper assay determination cellulase activity Appl Biochem Biotechnol127 Apr Cited by: 1 article PMID: Xiao ZStorms RTsang A. Biotechnol Bioeng88 701 Dec Cited by: 98 articles PMID: Percival Zhang YHHimmel MEMielenz JR, automated filter paper assay determination cellulase activity.

Biotechnol Adv24 527 Mar Cited by: articles PMID: Dashtban MMaki MLeung KTMao CQin W. Crit Rev Biotechnol30 424 Sep Cited by: 73 articles PMID: Contact us. Europe PMC requires Javascript to function effectively. Recent Activity. Search life-sciences literature Over 39 million articles, preprints and more Search Quick link: Coronavirus articles and preprints. Recent history Saved searches.

Abstract Available from publisher site using DOI. A subscription may be required. Stephen R Decker National Bioenergy Center, National Renewable Energy Laboratory, Cole Boulevard, Golden, automated filter paper assay determination cellulase activity, COUSA.

Author profile Search articles by ORCID Decker SR 1. William S Adney Search articles by 'William S Adney'. Adney WS. Edward Jennings Search articles by 'Edward Jennings'. Jennings E. Todd B Vinzant Search articles by 'Todd B Vinzant'. Vinzant TB. Michael E Himmel Search articles by 'Michael E Himmel'.

Himmel ME. Affiliations 1 author 1. National Bioenergy Center, National Renewable Energy Laboratory, Cole Boulevard, Golden, COUSA. Share this article Share with email Share with twitter Share with linkedin Share with facebook. Abstract Recent developments in molecular breeding and directed evolution have promised great developments in industrial enzymes as demonstrated by exponential improvements in beta-lactamase and green fluorescent protein GFP.

Detection of and screening for improved enzymes are relatively easy if the target enzyme is expressible in a suitable high-throughput screening host and a clearly defined and usable screen or selection is available, as with GFP and beta-lactamase.

Fungal cellulases, however, are difficult to measure and have limited expressibility in heterologous hosts. Furthermore, traditional cellulase assays are tedious and time-consuming. Although the International Union of Pure and Applied Chemists standard measure of cellulase activity, the filter paper assay FPAcan be reproduced in most laboratories with some effort, this method has long been recognized for its complexity and susceptibility to operator error.

Our current automated FPA method is based on a Cyberlabs C robotics deck equipped with customized incubation, reagent storage, and plate-reading capabilities that allow rapid evaluation of cellulases acting on cellulose and has a maximum throughput of 84 enzyme samples per day when performing the automated FPA.

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Explore citation contexts and check if this article has been supported or disputed. Undefined cellulase formulations hinder scientific reproducibility. Automated chromatographic laccase-mediator-system activity assay. Anders NSchelden MRoth SSpiess AC Anal Bioanal Chem2002 Jun Cited by: 0 articles PMID: Cellulolytic RoboLector - towards an automated high-throughput screening platform for recombinant cellulase expression.

Cellulases: Classification, automated filter paper assay determination cellulase activity, Methods of Determination and Industrial Applications.

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A simple and fast method for the determination of endo- and exo-cellulase activity in cellulase preparations using filter paper. Luciano Silveira MHRau MPinto da Silva Bon EAndreaus J Enzyme Microb Technol51 531 Jul Cited by: 3 articles PMID: A simplified filter paper assay method of cellulase enzymes based on HPLC analysis. Chu DDeng HZhang XZhang JBao J Appl Biochem Biotechnol127 Apr Cited by: 1 article PMID: Microplate-based filter paper assay to measure total cellulase activity.

Xiao ZStorms RTsang A Biotechnol Bioeng88 701 Dec Cited by: 98 articles PMID: Outlook for cellulase improvement: screening and selection strategies. Percival Zhang YHHimmel MEMielenz JR Biotechnol Adv24 527 Mar Cited by: articles PMID: Review. Cellulase activities in biomass conversion: measurement methods and comparison.

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Automated Filter Paper Assay for Determination of Cellulase Activity Automated FISH spot counting in interphase nuclei: Statistical validation and data correction Automated Filtering of Network Intrusion Detection Alarms Jan 01,  · The most common total cellulase activity assay is the filter paper assay (FPA) established by the International Union of Pure and Applied Chemistry (IUPAC). A new procedure to measure the FPA with microplate-based assay was studied in this work, which followed the main idea of IUPAC to dilute cellulase preparation to get fixed glucose blogger.com by: 23 assays and sample values are corrected for any blank values. 2. Scope. This procedure is only appropriate for the determination of FPU activity in a cellulase preparation as defined by the IUPAC procedure as outlined above. 3. References. Ghose, T.K. "Measurement of Cellulase Activities." Pure & Appl. Chem.